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KMID : 0604520100360040303
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Journal of the Society of Cosmetic Scientists of Korea
2010 Volume.36 No. 4 p.303 ~ p.314
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Antibacterial, Antioxidative Activity and Component Analysis of Pinus koraiensis Leaf Extracts
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Kim Jung-Eun
Kim Woo-Yeon
Kim Ji-Wook
Park Hyun-Soo
Lee Seung-Hoon
Lee Soon-Young
Kim Min-Ji
Kim A-Reum
Park Soo-Nam
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Abstract
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In this study, the antibacterial, antioxidative effect and component analysis of Pinus koraiensis leaf extracts were investigated. MIC values of the ethyl acetate fraction from P. koraiensis leaf extracts on P. acnes, S. aureus, P. ovale, and E. coli were 0.06 %, 0.25 %, 0.13 % and 0.50 %, respectively. The results showed that the antibacterial activity of the ethyl acetate fraction on P. acnes, P. ovale. and S. aureus was more prominent. The aglycone fraction of P. koraiensis leaf extracts () showed more higher free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (). Reactive oxygen species (ROS) scavenging activity () of P. koraiensis leaf extracts on ROS generated in -EDTA/ system was investigated using the luminol-dependent chemiluminescence assay. The 50 % ethanol extract () showed the most prominent ROS scavenging activity. Also the ethyl acetate () and the aglycone fraction () showed very high antoxidant activity. The protective effects of extract/fractions of P. koraiensis leaf extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The P. koraiensis leaf extracts showed cellular membrane protective effects in a concentration dependent manner (). TLC and HPLC chromatogram of the ethyl acetate fraction obtained from hydrolysis of P. koraiensis leaf extracts revealed 2 main bands (PK-4, PK-6) and peaks (peak 1, peak 2), which were identified as kaempferol-3-O-glucoside (PK-6, peak 1) and kaempferol-3-O-arabinoside (PK-4, peak 2) by LC/ESI-MS/MS, respectively. These results indicate that extract/fractions of P. koraiensis can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging ROS, and protect cellular membrane against ROS. Extract/fractions of P. koraiensis can be applicable to new cosmeceuticals for antioxidant, antiaging, and antibacterial activity.
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KEYWORD
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Pinus koraiensis leaf, antibacterial activity, antioxidative activity, LC/ESI-MS/MS, component analysis
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